CBSE 12th Class Biotechnology Syllabus

Course Structure

Units	Topics	Marks
V	Protein & Gene Manipulation	40
VI	Cell Culture & Genetic Manipulation	30
Practical		30
Total		100

Course Syllabus

Unit V: Protein and Gene Manipulation

Chapter 1: Recombinant DNA Technology

• Introduction
• Tool of rDNA technology
• Making rDNA
• Introduction of recombinant DNA into host cells
• Identification of recombinants
• Polymerase chain reaction (PCR)
• Hybridization techniques
• DNA library
• DNA sequencing
• Site-directed mutagenesis

Chapter 2: Protein Structure and Engineering

• Introduction to the world of proteins
• 3-D shape of proteins
• Structure-function relationship in protein
• Purification of proteins
• Characterization of proteins
• Protein based products
• Designing proteins (protein engineering)

Chapter 3: Genomics and Bioinformatics

• Introduction
• Genome sequencing projects
• Gene prediction and counting
• Genome similarity
• SNPs and comparative genomics
• Functional genomics
• Proteomics
• History of bioinformatics
• Sequences and nomenclature
• Information sources
• Analysis using bioinformatics tools

Unit VI: Cell Culture and Genetic Manipulation

Chapter I: Microbial Culture and Applications

• Introduction
• Microbial culture techniques
• Measurement and kinetics of microbial growth
• Scale up of microbial process
• Isolation of microbial products
• Strain isolation and improvement
• Applications of microbial culture technology
• Biosafety issues in microbial technology

Chapter II: Plant Cell Culture and Applications

• Introduction
• Cell and tissue culture techniques
• Applications of cell and tissue culture
• Gene transfer methods in plants
• Transgenic plants with beneficial traits
• Biosafety in plant genetic engineering

Chapter III: Animal Cell Culture and Applications

• Introduction
• Animal cell culture techniques
• Characterisation of cell lines
• Methods of gene delivery into cells
• Scale-up of animal culture process
• Applications of animal cell culture
• Stem cell technology
• Tissue engineering

Practical Works

List of Experiments

• Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
• Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
• Bacterial transformation using any plasmid
• Data retrieval and data base search using internet site NCBI
• Download a DNA and protein sequence from internet, analyse it and comment on it
• Cell viability assay
• Determination of blood groups
• Estimation of DNA
• Ion-exchange chromatography for proteins
• Reading of DNA sequencing gel to arrive at the sequence
• Estimation of blood glucose by enzymatic method (GOD/POD)
• Project work